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. 2024 May 22;138(10):599–614. doi: 10.1042/CS20231752

Figure 2. In situ hybridization for miR-486-5-p in rat kidney cortex.

Figure 2

Localization of miR-486-5p in the rat kidney cortex 24 h post-IR, with injection of miR-486-5p (0.5 mg/kg, IR+miR-486-5p) or scramble miRNA (0.5 mg/kg, IR+scb) at the time of reperfusion. At 24 h, enhanced miR-486-5p signal (pink-red areas) was observed in rats injected with miR-486-5p, localized to proximal tubular cells (arrow) and endothelial cells (asterisk). Background nuclear staining with hematoxylin. Scale bar = 10 µm, magnification ×1000. The graph shows the quantification of miR-486-5p probe copies/field of view (++++P<0.0001 vs all groups)