FIG. 2.

Sequence of the promoter regions from flaB and flgB of B. burgdorferi and the kan gene of Tn903. The NgoMIV site was added to the 5′ end of the flaB and flgB promoters for cloning. The underlined sequence denotes the NdeI fusion site between the promoters and the kan gene. The nucleotides shown in boldface within the underlined sequence differ from the wild-type sequence. The transcription initiation sites are indicated by arrows, and the proposed −10 and −35 sequences of the promoters are overlined (8, 9, 11, 14, 15). The first five amino acids of the kan product are shown below their corresponding codons.