TABLE 2.
Oligonucleotide | Sequencea | Application |
---|---|---|
1. kan 5′ + NdeI | CATATGAGCCATATTCAACGGGAAACG | 5′ kan fusion with Borrelia promoter |
2. pOK.3 | GATCGCCCTTCCCAACAGTTGC | 3′ end of kan gene 3. |
3. flaB 5′ prom + NotI | TGTCTGTCGCCTCTTGCGGCCGCCGGAGGAG | 5′ end of flaB promoter |
4. flaB 3′ prom + NdeI | GATTGATAATCATATGTCATTCCTCCATG | flaB promoter 3′ fusion with kan |
5. flgB 5′ prom | TAATACCCGAGCTTCAAGGAAG | 5′ end of flgB promoter |
6. flgB 3′ prom + NdeI | CTTTCAAAATCATTCATATGGAAACCTCCCTC | flgB promoter 3′ fusion with kan |
7. flaB 5′ prom + NgoMIV | GCCGGCTGTCTGTCGCCTCTTGTGGCTTCCGG | pJLB 500A |
8. flgB 5′ prom + NgoMIV | GCCGGCTAATACCCGAGCTTCAAGGAG | pJLB 500G |
9. pOK.7 + NheI | GGCGAATGAGCTAGCGCCGTCCC | pJLB 500A and -G |
10. pOK.8 + NheI | GGGACGGCGCTAGCTCATTCGCC | pJLB 500A and -G |
11. pOK.9 + NgoMIV | GCCGGCGACAGTTTTATTGTTCATGATG | pJLB 500A and -G |
12. pOK.6 | ATGCAAAAGCACCACTGGCAGC | Sequencing promoter fusions |
13. FL-6 | TTCAGGGTCTCAAGCGTCTTGGACT | flaB gene-specific probe |
14. FL-7 | GCATTTTCAATTTTAGCAAGTGATG | flaB gene-specific probe |
15. oppA.76 | CGTGCTCAGCAATGTCTAAAC | 5′ ΔoppAV |
16. oppA.83 | CATGTTTCAGGCTCAATTTCTAC | 3′ ΔoppAV |
17. pOK12-Rev2 | TGTGGAATTGTGAGCGGATAAC | Screen for oppAV integrants |
18. oppA.73 | GCGTCTTTTTAAGCCTTTTGCC | Screen for oppAV integrants |
Restriction sites in boldface.