TABLE 2.

Oligonucleotides (5′→3′) used in this study

Oligonucleotide	Sequencea	Application
1. kan 5′ + NdeI	CATATGAGCCATATTCAACGGGAAACG	5′ kan fusion with Borrelia promoter
2. pOK.3	GATCGCCCTTCCCAACAGTTGC	3′ end of kan gene 3.
3. flaB 5′ prom + NotI	TGTCTGTCGCCTCTTGCGGCCGCCGGAGGAG	5′ end of flaB promoter
4. flaB 3′ prom + NdeI	GATTGATAATCATATGTCATTCCTCCATG	flaB promoter 3′ fusion with kan
5. flgB 5′ prom	TAATACCCGAGCTTCAAGGAAG	5′ end of flgB promoter
6. flgB 3′ prom + NdeI	CTTTCAAAATCATTCATATGGAAACCTCCCTC	flgB promoter 3′ fusion with kan
7. flaB 5′ prom + NgoMIV	GCCGGCTGTCTGTCGCCTCTTGTGGCTTCCGG	pJLB 500A
8. flgB 5′ prom + NgoMIV	GCCGGCTAATACCCGAGCTTCAAGGAG	pJLB 500G
9. pOK.7 + NheI	GGCGAATGAGCTAGCGCCGTCCC	pJLB 500A and -G
10. pOK.8 + NheI	GGGACGGCGCTAGCTCATTCGCC	pJLB 500A and -G
11. pOK.9 + NgoMIV	GCCGGCGACAGTTTTATTGTTCATGATG	pJLB 500A and -G
12. pOK.6	ATGCAAAAGCACCACTGGCAGC	Sequencing promoter fusions
13. FL-6	TTCAGGGTCTCAAGCGTCTTGGACT	flaB gene-specific probe
14. FL-7	GCATTTTCAATTTTAGCAAGTGATG	flaB gene-specific probe
15. oppA.76	CGTGCTCAGCAATGTCTAAAC	5′ ΔoppAV
16. oppA.83	CATGTTTCAGGCTCAATTTCTAC	3′ ΔoppAV
17. pOK12-Rev2	TGTGGAATTGTGAGCGGATAAC	Screen for oppAV integrants
18. oppA.73	GCGTCTTTTTAAGCCTTTTGCC	Screen for oppAV integrants

^aRestriction sites in boldface.