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(A) Architecture of ALK. (B) Schematic of protein biotinylation in the BirA enzyme expression cell. The protein was biotinylated in the endoplasmic reticulum (ER) by ER–retained biotin ligase (BirA-ER). After folding and modification, the biotinylated protein was then secreted into the medium. (C) ELISA results of biotin-labeled ALK antigen. Compared with M336 control, all purified ALK domains were successfully biotinylated. (D) Quantification of ELISA results from (C).
