TABLE 1.

DNA probes used in this study

Borrelia plasmid source	DNA probea	Locationb	ORFs in probeb
lp5	CB63c	3413–5195	T05–T06
lp17	CL47	63–2418	D01–D04
	D11d	6949–7679	D11
lp21	CZ32	6788–8697	63-bp repeat region
	DF29c	16772–18682	U10–U12
lp25	DE21	13151–14265	E20–E21
	CG18	19568–21587	E29
lp28-1	DD60	5149–6980	F11–F13
	EN57c	24797–26510	vlsE cassettes
lp28-2	DK44	1–442	G01
	EI58	6700–8688	G09–G10
lp28-3	CT53c	3659–4392	H08–H09
	CQ68	12620–14584	H18–H20
lp28-4	EE50	2526–3627	I06–I07
	CV69	4726–6439	I11–I14
	EL95	17047–19017	I27–I29
lp36	DK60	2334–4214	K02–K04
	FI78	7569–9698	K12–K15
	CQ33c	12657–15072	K19–K22
	GI88	26201–26513	K41
	EH86	31975–33745	K49–K50
lp38	BA19c	22–1483	J001
	DH08	3958–6033	J07–J08
	CQ63	13730–16123	J20–J22
	DH46	21666–23409	J28–J30
	FA84	26057–28222	J34–J36
	CT79c	35768–37561	J48–J50
lp54	CM64	427–2449	A01–A04
	A24/25d	∼15800–17300	A24–A25 (dbpAB)
lp56	BL05	3022–3666	Q05–Q06
	EK58	42201–44134	Q67–Q69

^aDNA clone numbers from genome sequencing project (8, 12). 

^bBase pair location and open reading frame (ORF) nomenclature are as in Fraser et al. (12) and Casjens et al. (8). 

^cShowed some weak hybridization to other plasmids when probing B31 MI DNA. 

^dUncloned PCR product made with whole B. burgdorferi B31 MI DNA as template.