TABLE 2.
Sizes of hybridizing restriction fragments
Probe used | Enzyme tested | Fragmenta (kb) | Presence of fragment in strainb:
|
|||||
---|---|---|---|---|---|---|---|---|
MG1363 | NCDO712 | NCDO763 | NCDO2031 | LM0230 | LM2301 | |||
ISS1 | SmaI | 130 [Sm6] | + | + | + (160) | + | + | + (105) |
55 | − | + | − | + | − | − | ||
ApaI | 75 [Ap10C] | + | + | + | + | + | + (120) | |
55 | − | + | + | + | − | − | ||
φT712 | SmaI | 220* [Sm4] | + | + | + | + | + | + |
180* [Sm5] | + | − | + | − | + | + | ||
105 [Sm8] | + | + | + | + | − | − | ||
22 [Sm14] | + | + | + | + | + | + | ||
ApaI | 165* | − | + | − | + | − | − | |
160* [Ap3] | + | − | + | − | + | + | ||
49 [Ap14] | + | + | + | + | − | − | ||
13 [Ap27] | + | + | + | + | − | − | ||
12 [Ap28] | + | + | + | + | − | − | ||
33 [Ap19] | + | + | + | + | + | + | ||
Sm8 | SmaI | 105 [Sm8] | + | + | + | + | + (58) | + (58) |
ApaI | 205 [Ap1] | + | + | + | + | + | + | |
49 [Ap14A] | + | + | + | + | + (29) | + (29) | ||
13 [Ap27B] | + | + | + | + | − | − | ||
12 [Ap28] | + | + | + | + | − | − | ||
8 [Ap29] | + | + | + | + | + | + | ||
Ap3 | SmaI | 180 [Sm5A] | + | + (220) | + | + (220) | + | + |
ApaI | 160 [Ap3] | + | + (165) | + | + (165) | + | + | |
No2 | NotI | 230 [No2] | + | + | + (200) | + (175) | + (175) | + (175) |
SmaI | 610 [Sm1] | + | + | + (580) | + (550) | + (550) | + (550) | |
ApaI | 205 [Ap1] | + | + | + | + | + | + | |
145 [Ap4] | + | + | + (120) | + (100) | + (100) | + (100) | ||
16 [Ap26] | + | + | + | − | − | − | ||
5′-cluA | SmaI | 610 [Sm1] | + | + | + (580) | − | − | − |
ApaI | 50* | + | + | + (25) | − | − | − | |
16 [Ap26] | + | + | + | − | − | − | ||
5′-oppD | SmaI | 130 [Sm6] | + | + | + (160) | + | + | − |
55 | − | + | − | + | + | − | ||
ApaI | 75 [Ap10C] | + | + | + | + | + | − | |
69* [Ap11] | + | + | + (19) | + | + (67) | − | ||
55 | − | + | − | + | + (65) | − | ||
oppFBc | SmaI | 130 [Sm6] | + | + | + (160) | + | + | − |
55 | − | + | − | + | + (58) | − | ||
ApaI | 75 [Ap10C] | + | + | + | + | + | − | |
55 | − | + | − | + | + (65) | − |
Fragment names are given in brackets. Asterisks indicate a weak hybridization signal. Sizes different from the size given in this column are shown in parentheses.
NCDO712 refers to NCDO712 and NCDO505 genomes; NCDO763 refers to NCDO763 and MMS36 genomes; and NCDO2031 refers to NCDO2031 and C2 genomes.
Probes oppCA and pepO gave the same hybridization pattern as oppFB.