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. 2000 May;182(9):2481–2491. doi: 10.1128/jb.182.9.2481-2491.2000

TABLE 2.

Sizes of hybridizing restriction fragments

Probe used Enzyme tested Fragmenta (kb) Presence of fragment in strainb:
MG1363 NCDO712 NCDO763 NCDO2031 LM0230 LM2301
ISS1 SmaI 130 [Sm6] + + + (160) + + + (105)
55 + +
ApaI 75 [Ap10C] + + + + + + (120)
55 + + +
φT712 SmaI 220* [Sm4] + + + + + +
180* [Sm5] + + + +
105 [Sm8] + + + +
22 [Sm14] + + + + + +
ApaI 165* + +
160* [Ap3] + + + +
49 [Ap14] + + + +
13 [Ap27] + + + +
12 [Ap28] + + + +
33 [Ap19] + + + + + +
Sm8 SmaI 105 [Sm8] + + + + + (58) + (58)
ApaI 205 [Ap1] + + + + + +
49 [Ap14A] + + + + + (29) + (29)
13 [Ap27B] + + + +
12 [Ap28] + + + +
8 [Ap29] + + + + + +
Ap3 SmaI 180 [Sm5A] + + (220) + + (220) + +
ApaI 160 [Ap3] + + (165) + + (165) + +
No2 NotI 230 [No2] + + + (200) + (175) + (175) + (175)
SmaI 610 [Sm1] + + + (580) + (550) + (550) + (550)
ApaI 205 [Ap1] + + + + + +
145 [Ap4] + + + (120) + (100) + (100) + (100)
16 [Ap26] + + +
5′-cluA SmaI 610 [Sm1] + + + (580)
ApaI 50* + + + (25)
16 [Ap26] + + +
5′-oppD SmaI 130 [Sm6] + + + (160) + +
55 + + +
ApaI 75 [Ap10C] + + + + +
69* [Ap11] + + + (19) + + (67)
55 + + + (65)
oppFBc SmaI 130 [Sm6] + + + (160) + +
55 + + + (58)
ApaI 75 [Ap10C] + + + + +
55 + + + (65)
a

Fragment names are given in brackets. Asterisks indicate a weak hybridization signal. Sizes different from the size given in this column are shown in parentheses. 

b

NCDO712 refers to NCDO712 and NCDO505 genomes; NCDO763 refers to NCDO763 and MMS36 genomes; and NCDO2031 refers to NCDO2031 and C2 genomes. 

c

Probes oppCA and pepO gave the same hybridization pattern as oppFB