TABLE 1.
B. subtilis strains
| Strain | Genotype (antibiotic resistance) | Sourcea |
|---|---|---|
| PS832 | Wild typeb | Laboratory stock |
| FB20 | ΔgerA::spc | pFE11→PS832 |
| FB60 | ΔgerB::cat | pFE107→PS832 |
| FB68 | ΔgerK::ermC | pFE143→PS832 |
| FB77 | ΔyndDEF::tet | pFE170→PS832 |
| FB83 | ΔyfkQRT::cat | pFE168→PS832 |
| FB84 | ΔyfkQRT::neo | pCm::Km→FB83 |
| FB61 | ΔgerA::spc ΔgerB::cat | FB60 DNA→FB20 |
| FB86 | ΔgerA::spc ΔgerK::ermC | FB68 DNA→FB20 |
| FB87 | ΔgerB::cat ΔgerK::ermC | FB68 DNA→FB60 |
| FB72 | ΔgerA::spc ΔgerB::cat ΔgerK::tet | FB68 DNA→FB61 |
| FB85 | ΔgerA::spc ΔgerB::cat ΔgerK::ermC ΔyndDEF::tet ΔyfkQRT::neo | FB77 and FB84 DNA→FB72 |
a
The construction and/or source of the individual plasmids is described in Materials and Methods.
b
The genetic background is 168, but this strain is trp+.