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. 2024 Apr 2;11(20):2302379. [Article in Italian] doi: 10.1002/advs.202302379

Figure 4.

Figure 4

Characterization of mRNA 5‐methylcytosine (m5C) in ESCC. A) The mRNAs were extracted by two successive rounds of poly (A) purification using magnetic oligo d(T) beads from total RNAs. The levels of m5C in purified mRNA between ESCC tissues and adjacent normal esophageal tissues were measured by ELISA (n=13). B) The silencing and overexpression efficiency of NSUN2 was detected by western blot. C) m5C level in purified mRNA of ESCC cell lines with NSUN2 knockdown or overexpression was determined by ELISA. D) The m5C distributions within different regions in control and NSUN2 knockdown cells. E) The top consensus m5C motif in in control and NSUN2 knockdown cells. F) Distribution of mRNAs with a significant change in both the m5C methylation and gene expression levels in KYSE150 cells with NSUN2 knockdown. G) Pathway analysis of genes in cells with NSUN2 knockdown.