Figure 6.

YBX1 assists ESCC cell proliferation, migration, and invasion partially through upregulating SMOX. A) The SMOX level was assessed through a western blot assay in ESCC cells co‐transfected with blank vector & sh‐NC, vector & shYBX1‐2, shNC & oe‐SMOX, or shYBX1‐2 & oe‐SMOX. B‐D) CCK8 and colony formation assay determined cell proliferation ability of YBX1 knockdown or control ESCC cells after co‐transfected with SMOX. E,F) Cell migration and invasion ability were evaluated by transwell assay and were further quantified. G) Sphere formation ability of scramble and shYBX1‐2 & oe‐SMOX co‐transfected cells. ^* p < 0.05, ^** p < 0.01, and ^*** p < 0.001.