TABLE 1.
Purification of the native AlaAT and rAlaAT from P. furiosus and E. coli
| Purification step | Activity (U) | Protein (mg) | Sp act (U/mg) | Recovery (%) | Relative purification |
|---|---|---|---|---|---|
| AlaAT | |||||
| Cell extract | 8,928 | 2,880 | 3.1 | 100 | 1 |
| Q-Sepharose | 2,940 | 773 | 3.8 | 33 | 1.2 |
| Phenyl-Sepharose | 2,945 | 427 | 6.8 | 33 | 2.2 |
| Hydroxyapatite | 2,992 | 60 | 50 | 33 | 16 |
| S-Sepharose | 1,984 | 44 | 45 | 22 | 15 |
| Mono-Q | 1,411 | 14 | 101 | 16 | 33 |
| Superdex 200 | 616 | 3 | 158 | 7 | 51 |
| rAlaAT | |||||
| Cell extract | 5,660 | 396 | 14.2 | 100 | 1 |
| Heat incubation | 4,400 | 25.8 | 170 | 78 | 12 |
| Mono-Q | 2,386 | 9.8 | 243 | 42 | 17 |