TABLE 2.
Kinetic parameters of P. furiosus AlaAT and rAlaATa
| Substrate pair | Apparent Km (mM) | Apparent Vmax (μmol/min/mg) | kcat (s−1) | kcat/Km (s−1 mM−1) |
|---|---|---|---|---|
| AlaAT | ||||
| Alanine | 2.8 | 151 | 114 | 41 |
| α-Ketoglutarate | 0.9 | |||
| Glutamate | 4.3 | 187 | 142 | 33 |
| Pyruvate | 5.4 | |||
| rAlaAT | ||||
| Alanine | 3.2 | 245 | 186 | 58 |
| α-Ketoglutarate | 1.1 | |||
| Glutamate | 4.7 | 285 | 216 | 46 |
| Pyruvate | 5.6 |
a
Activities were determined at 80°C as described in the Materials and Methods. For alanine and glutamate, 5 mM α-ketoglutarate and pyruvate were used as the amino acceptors. For α-ketoglutarate and pyruvate, 20 mM alanine and glutamate were used as the amino donors.