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Substrate specificity of rAlaAT toward various amino acids with α-ketoglutarate or pyruvate as the amino acceptor
| Amino acid | Relative activity (%)a
|
|
|---|---|---|
| α-Ketoglutarate | Pyruvate | |
| Alanine | 100 | |
| Glutamate | 100 | |
| Aspartate | 14 | 10 |
| Valine | 4 | <0.1 |
| Isoleucine | 4 | <0.1 |
| Leucine | 4 | <0.1 |
| Tyrosine | <0.1 | <0.1 |
| Phenylalanine | <0.1 | <0.1 |
For α-ketoglutarate, 100% activity is 150 U/mg, and for pyruvate, 100% activity is 180 U/mg.
