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SDS-PAGE analysis of LPSs directed by wbdA with and without the R55C mutation. A silver-stained gel (A) and an immunoblot of the same gel using the O9a MAb (B) are shown. LPSs examined were from E. coli HU1190(pNKB26::Tn1000-52) carrying the following plasmids: pBSC25-1 (lane 1), pBSC25-1 (R55C) (lane 2), pWBDA-FB (lane 3), and pWBDA-FB (R55C) (lane 4).
