Fig 1. Many non-canonical genetic parts are found on plasmids.

(A) Overall representation in Addgene plasmids of genetic part variants with sequences that differ slightly from canonical features present in annotation databases. Within each part type, the total number of genetic parts (green squares), total number of genetic parts that are variants (i.e., differ from the canonical sequence) (orange circles), and number of distinct genetic part variant sequences (i.e., counting each unique sequence that differs from the canonical sequence one time) (blue triangles) are plotted. Part types are sorted in descending order by the number of total variants in each category. (B) Distributions of percent identity between distinct genetic part variants in each category and their canonical sequences. Boxes represent lower and upper quartiles (the interquartile range). Vertical lines within each box are medians. The whiskers correspond to 1.5 times the interquartile range. Points are outliers outside this range.