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. 2024 Apr 19;13:e89157. doi: 10.7554/eLife.89157

Figure 5. Histopathological analysis of lung samples.

Figure 5.

(A) Histological haematoxylin and eosin (H&E) staining of lung samples at week 4 post Mtb challenge. Scale bar = 2.5 mm. (B) Analysis of percentage of inflamed area (indicated with black boxes) from each mouse lung per immunized group (n=5 per group), shows that rBCG::iE-DAP+Dox immunized mice present with early lung inflammation compared to WT BCG+Dox. (C) H&E staining of lung samples at week 8 post Mtb H37Rv infection. Scale bar = 2.5 mm. (D) Analysis of percentage of inflamed area from each mouse lung (n=5 per group). The percentage inflamed area was evaluated using ImageJ software (NIH) and plotted as whisker box-plots (whiskers represent minimum and maximum values) and a student t-test was used for statistical analysis. Lung sections were derived from 5 mice per group (n=5) from Figure 4a experiments. Statistical analysis was conducted using student t-test. The error bars represent the standard deviation relative to the mean. *: p-value <0.05.

Figure 5—source data 1. Histopathological analysis of lung samples.