Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background Mus musculus | C57BL/6J | Jackson Laboratory | Stock# 000664; RRID:IMSR_JAX:000664 | Sex: Male (because B16 cell line is male derived) |
| Strain, strain background Mus musculus (male) | NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | Perelman School of Medicine Stem Cell Xenograft Core | RRID:SCR_010035 | Sex: Male |
| Cell line (Mus musculus) | B16F10 | ATCC | Cat# CRL-6475; RRID:CVCL_0159 | Mouse melanoma |
| Cell line (Mus musculus) | B16F10 CD47 KO | PMID: 31964705 | CD47 KO | Hayes et al., 2020 |
| Cell line (Mus musculus) | B16F10 sgCtrl | PMID: 31964705 | sgCtrl | Hayes et al., 2020 |
| Biological sample (Mus musculus) | C57BL/6J bone marrow cells | This paper | Flushed from femurs, tibias; differentiation into bone marrow-derived macrophages (BMDMs) or adoptive transfer | |
| Biological sample (Mus musculus) | C57BL/6J serum | This paper | Retro-orbital blood collected from convalescent or naïve mice, clotted for 1 hr, separated by centrifugation | |
| Antibody | InVivoMab mouse anti-human/mouse Tryp1 (clone TA99) | BioXCell | Cat# BE0151; RRID:AB_10949462 | (1:1000) Opsonization (10–20 μg/mL) In vivo (250 μg/100 μL per dose) |
| Antibody | InVivoMab mouse IgG2a isotype control (clone C1.18.4) | BioXCell | Cat# BE0085; RRID:AB_1107771 | (1:1000) (10–20 μg/mL) In vivo (250 μg/100 μL per dose) |
| Antibody | Ultra-LEAF rat anti-mouse SIRPα (clone P84) | BioLegend | Cat# 144036; RRID:AB_2832517 | Blockade (18 μg/mL=1:50) |
| Antibody | InVivoMab rat anti-mouse CD47 (clone MIAP301) | BioXCell | Cat# BE0270; RRID:AB_2687793 | (1:500) Flow cytom. (20 μg/mL) |
| Antibody | Alexa Fluor 647 anti-mouse IgG [H+L] (donkey polyclonal) | Thermo Fisher Invitrogen | Cat# A-31571; RRID:AB_162542 | Flow cytom. (10 μg/mL=1:200) |
| Antibody | Alexa Fluor 647 anti-rat IgG [H+L] (goat polyclonal) | Thermo Fisher Invitrogen | Cat# A-21247; RRID:AB_141778 | Flow cytom. (10 μg/mL=1:200) |
| Antibody | Horse radish peroxidase sheep anti-mouse [H+L], polyclonal | GE Life Sciences | Cat# NA931V | WB (1:500) |
| Antibody | Mouse anti-β-actin (clone C4) | Santa Cruz | Cat# sc47778; RRID:AB_626632 | WB (1:1000) |
| Antibody | Brilliant Violet 650 rat anti-mouse CD45 (clone 30-F11) | BioLegend | Cat# 103151; RRID:AB_2565884 | Flow cytom. (2.5 µg/mL=1:80) |
| Antibody | Brilliant Violet 785 rat anti-mouse CD45 (clone 30-F11) | BioLegend | Cat# 103149; RRID:AB_2564590 | Flow cytom. (5 µg/mL=1:40) |
| Antibody | APC/Cyanine7 rat anti-mouse CD45 (clone 30-F11) | BioLegend | Cat# 103115; RRID:AB_312980 | Flow cytom. (2.5 µg/mL=1:80) |
| Antibody | APC rat anti-mouse/human CD11b (clone M1/70) | BioLegend | Cat# 101212; RRID:AB_312795 | Flow cytom. (2.5 µg/mL=1:80) |
| Antibody | PE/Cyanine7 rat anti-mouse/human CD11b (clone M1/70) | BioLegend | Cat# 101216; RRID:AB_312799 | Flow cytom. (2.5 µg/mL=1:80) |
| Antibody | PE/Dazzle 594 rat anti-mouse Ly6G (clone 1A8) | BioLegend | Cat# 127647; RRID:AB_2566318 | Flow cytom. (5 µg/mL=1:40) |
| Antibody | PerCP rat anti-mouse Ly6G (clone 1A8) | BioLegend | Cat# 127653; RRID:AB_2616998 | Flow cytom. (2.5 µg/mL=1:80) |
| Antibody | PE rat anti-mouse F4/80 (clone BM8) | BioLegend | Cat# 123110; RRID:AB_893486 | Flow cytom. (10 µg/mL=1:20) |
| Antibody | Brilliant Violet 605 rat anti-mouse Ly-6C (clone HK1.4) | BioLegend | Cat# 128035 RRID:AB_2562352 | Flow cytom (5 µL/100 µL=1:20) |
| Antibody | APC rat anti-mouse I-A/I-E (clone M5/114.15.2) | BioLegend | Cat# 107614; RRID:AB_313329 | Flow cytom. (2.5 µg/mL=1:80) |
| Antibody | Pacific Blue rat anti-mouse CD86 (clone GL-1) | BioLegend | Cat# 105022; RRID:AB_493466 | Flow cytom. (10 µg/mL=1:50) |
| Antibody | APC/Cyanine7 rat anti-mouse CD86 (clone GL-1) | BioLegend | Cat# 105029; RRID:AB_2074993 | Flow cytom. (2.5 µg/mL=1:80) |
| Antibody | APC rat anti-mouse CD206 (clone C068C2) | BioLegend | Cat# 141707; RRID:AB_10896057 | Flow cytom. (5 µg/mL=1:40) |
| Antibody | Brilliant Violet 421 rat anti-mouse CD206 (clone C068C2) | BioLegend | Cat# 141717; RRID:AB_2562232 | Flow cytom. (5 µL/100 µL=1:20) |
| Antibody | PE/Cyanine7 rat anti-mouse CD163 (clone S15049F) | BioLegend | Cat# 156707; RRID:AB_2910324 | Flow cytom. (2.5 µg/mL=1:80) |
| Antibody | APC/Cyanine7 Armenian hamster anti-mouse CD3e (clone 145-2C11) | BioLegend | Cat# 100329; RRID:AB_1877171 | Flow cytom. (5 µg/mL=1:40) |
| Antibody | Alexa Fluor 647 rat anti-mouse CD8a (clone 53-6.7) | BioLegend | Cat# 100727; RRID:AB_493424 | Flow cytom. (2.5 µg/mL=1:200) |
| Antibody | APC mouse anti-mouse H-2kb/H-2Db (clone 28-8-6) | BioLegend | Cat# 114613; RRID:AB_2750193 | Flow cytom. (5 µg/mL=1:40) |
| Antibody | PE rat anti-mouse IgG2a (clone RMG2a-62) | BioLegend | Cat# 407108; RRID:AB_10549974 | Flow cytom. (5 µg/mL=1:40) |
| Antibody | APC rat anti-mouse IgG2b (clone RMG2b-1) | BioLegend | Cat# 406711; RRID:AB_2750277 | Flow cytom. (2.5 µg/mL=1:80) |
| Antibody | TruStain FcX PLUS rat anti-mouse CD16/32 (clone S17011E) | BioLegend | Cat# 156603; RRID:AB_2783137 | Flow cytom. Fc block (2.5 µg/mL=1:200) |
| Peptide, recombinant protein | Macrophage colony-stimulating factor (M-CSF) | BioLegend | Cat# 576406 | BMDM differentiation (20 ng/mL) |
| Commercial assay or kit | Vybrant CFDA-SE Cell Trace | Thermo Fisher Invitrogen | Cat# V12883 | |
| Commercial assay or kit | CellTracker DeepRed | Thermo Fisher Invitrogen | Cat# C34565 | |
| Commercial assay or kit | NEBNext Ultra II RNA Library Prep Kit | NEB | Cat# E7770S | |
| Sequence-based reagent | NEBNext Multiplex Oligos for Illumina | NEB | Cat# E7335 | |
| Commercial assay or kit | Chromium Single Cell Gene Expression kit | 10x Genomics | Cat# PN-1000128; Cat# PN-1000127; Cat# PN-1000213 | |
| Chemical compound, drug | Reversine | Cayman Chemical | Cat# 10004412 | MPS1 inhibitor |
| Chemical compound, drug | AZ3146 | Cayman Chemical | Cat# 19991 | MPS1 inhibitor |
| Chemical compound, drug | BAY 12-17389 | Selleck Chemicals | Cat# S8215 | MPS1 inhibitor |
| Software, algorithm | Prism v9.4 | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | FCS Express 7 | De Novo Software | ||
| Other | Anti-adherence rinsing solution | StemCell Technologies | Cat# 07010 | Surface treatment for tumoroid studies |
| Other | ACK lysing buffer | Thermo Fisher Gibco | Cat# A1049201 | Bone marrow and tumor red blood cell lysis |
| Other | Dispase | Corning | Cat# 354235 | Tumor disaggregation |
| Other | DNAse I | Millipore Sigma | Cat# 101041159001 | Tumor disaggregation |
| Other | Collagenase type IV | Thermo Fisher Gibco | Cat# 17104-019 | Tumor disaggregation (4 mg/mL) |