Figure 4. RNA-seq analysis indicated that deficiency of Cbfβ in cartilage reduces cell fate commitment, cartilage regeneration and repair, and increases canonical Wnt signaling and inflammatory response.
(A) Volcano plot showing differentially regulated gene expression in 6-week-old male Cbfbf/f and Cbfbf/f;Col2a1-CreERT mice hip articular cartilage. (B) Pie chart showing percentage of upregulated and downregulated differentially regulated genes in hip articular cartilage of 6-weeks-old male Cbfbf/f;Col2a1-CreERT mice compared to those of Cbfbf/f mice. The percentages of genes upregulated and downregulated are shown in red and green, respectively. (C) GO functional clustering of the top downregulated biological process (BP) in 6-week-old male Cbfbf/f;Col2a1-CreERT mice hip articular cartilage. (D) GO functional clustering of the top upregulated BP in 6-week-old male Cbfbf/f;Col2a1-CreERT mice hip articular cartilage. (E) GO functional clustering of the top downregulated KEGG signaling pathways in 6-week-old male Cbfbf/f;Col2a1-CreERT mice hip articular cartilage. (F) GO functional clustering of the top upregulated KEGG signaling pathways in 6-week-old male Cbfbf/f;Col2a1-CreERT mice hip articular cartilage.