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. 2024 May 28;13:e95640. doi: 10.7554/eLife.95640

Figure 8. Deficiency of Cbfβ protein levels increased β-catenin and articular cartilage degradation markers while also reducing Yap signaling activation.

(A) Western blot showing protein expression level of Yap in ATDC5 cells (n=3). (B) Quantification of Yap protein levels in (A). (C) Western blot of 10-week-old male hip cartilage from Cbfbf/f;Col2a1-CreERT mice injected with either oil or TMX showing the expression of Cbfβ, p-Smad2/3, Smad2/3, and Mmp13 (n=5). (D) Western blot of knee joint cartilage from 16-week-old male WT and Cbfbf/f;Col2a1-CreERT mice with ACLT surgery and injected with either oil or TMX showing the expression of Cbfβ (n=6). (E) Western blot of WT mice knee joint cartilage from 16-week-old male mice with ACLT surgery, treated with AAV-luc-YFP or AAV-Cbfb, and injected with either oil or TMX showing the expression of Cbfβ and active β-catenin (n=6). (F) Quantification of (C). (G) Quantification of (D). (H) Quantification of (E). Data are shown as mean ± SD. *p<0.05, **p<0.01, ***p<0.001. NS Not Significant.

Figure 8—source data 1. Labeled raw western blot data for Figure 8A (anti-Yap and anti-Gapdh).
Figure 8—source data 2. Unlabeled raw western blot data for Figure 8A (anti-Yap and anti-Gapdh).
Figure 8—source data 3. Labeled raw western blot data for Figure 8C (anti-Cbfβ, anti-p-Smad2/3, anti-Smad2/3, anti-Mmp13, and anti-Gapdh).
Figure 8—source data 4. Unlabeled raw western blot data for Figure 8C (anti-Cbfβ, anti-p-Smad2/3, anti-Smad2/3, anti-Mmp13, and anti-Gapdh).
Figure 8—source data 5. Labeled raw western blot data for Figure 8D (anti-Cbfβ and anti-Gapdh).
Figure 8—source data 6. Unlabeled raw western blot data for Figure 8D (anti-Cbfβ and anti-Gapdh).
Figure 8—source data 7. Labeled raw western blot data for Figure 8E (anti-Cbfβ, anti-active β-catenin, and anti-Gapdh).
Figure 8—source data 8. Unlabeled raw western blot data for Figure 8E (anti-Cbfβ, anti-active β-catenin, and anti-Gapdh).

Figure 8.

Figure 8—figure supplement 1. Alcian Blue staining of primary chondrocytes from Cbfβ deficient newborn mice show reduced matrix deposition.

Figure 8—figure supplement 1.

(A) Alcian Blue staining of newborn WT mouse primary chondrocytes. (B) Alcian Blue staining of newborn (P0) Cbfbf/fCol2a1-Cre mouse primary chondrocytes. (n=5).