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. 2024 May 28;13:e95640. doi: 10.7554/eLife.95640

Figure 9. Adeno-associated virus (AAV)-mediated Cbfb overexpression protects against ACLT mechanical OA.

Figure 9.

(A–B) X-ray images of the knee joints of 22-week-old male WT mice with ACLT surgery at 8-week-old with (A) AAV-YFP treatment and (B) AAV-Cbfb treatment (n=15). Yellow arrows indicates normal joint space; White arrows indicate worn articular cartilage; blue arrows indicate osteophytes; red arrows indicate joint space loss. (C–D) SO staining of knees from 16-week-old male WT mice with (C) AAV-YFP (control) or (D) AAV-Cbfb treatment in ACLT mediated OA (ACLT surgery at 8-week-old) (n=5). (E) Knee joint of OARSI score of (C) and (D). (F–G) X-ray images of mouse knee joints of 16-week-old male mice after sham/DMM surgery with (F) no treatment or (G) AAV-Cbfb treatment (n=15). White arrows: osteophytes and worn articular cartilage. (H–J) SO staining of knee joints of 16-week-old mice after sham/DMM surgery (DMM surgery at 8-week-old) with (H) Sham no treatment, (I) DMM surgery AAV-YFP treatment, or (J) AAV-Cbfb treatment (n=5). (K) Knee joint OARSI score of (H–J). The results are presented as the mean ± SD, *p<0.05, **p<0.01, ***p<0.001. DMM surgery AAV-YFP treatment group shows severe cartilage damage, osteophytes, and delocalized knee joint, while the AAV-Cbfb treated group shows less cartilage loss and osteophytes than control.