TABLE 1.
Polymer production and encystment in A. vinelandii strainsa
Strain | Alginate production (μg/mg of protein)
|
PHB production (μg/mg of protein) | Encystment (%) | ||
---|---|---|---|---|---|
BSb
|
BBc | ||||
Liquid | Solid | ||||
ATCC 9046 | 1,667 ± 11 | 1,246 ± 15 | 1,717 ± 11 | 413.1 ± 17.8 | 7.2 ± 1.5 |
ATCC 9046/pSMU588 | 666 ± 39 | NDd | ND | ND | ND |
ATCC 9046/pSMU588-21 | 1,320 ± 68 | ND | ND | ND | ND |
ATCC 9046/pMC15 | 16 ± 1.8 | ND | ND | 48.5 ± 0.5 | ND |
JM1 | 19 ± 1.5 | 411 ± 25 | 38 ± 2.8 | ND | <0.0001 |
JM2 | 36 ± 2.3 | 465 ± 53 | 29 ± 3.5 | 49.6 ± 0.9 | ND |
Alginate production was determined as previously described (21); all determinations were done in triplicate. PHB production was determined as previously described (29), in cells grown for 48 h in Burk's liquid medium supplemented with 2% sucrose. Values are means ± standard errors of the means.
BS, Burk's medium supplemented with 2% sucrose as the carbon source.
BB, Burk's medium supplemented with 0.2% n-butanol as the carbon source.
ND, not determined.