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. 2024 Apr 18;38(6):3212–3222. doi: 10.1007/s00464-024-10827-6

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© The Author(s) 2024

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The experimental operative and computational workflow. Curves denote time in seconds versus normalised fluorescence ratio. The photographs show still video frames with overlaid NIR and white light imagery from both (a and b) the reference and (c and d) determinative ICGFAs