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Decay of 32P-labeled RNAs. EG242 RNA and EG510 RNA were incubated with DEAE-Sepharose fractions from BG119 (pnpA) and BG296 (pnpA yvaJ). The samples contained either no divalent cation (lane 1), 1 mM Mg2+ (lane 2), or 1 mM Mn2+ (lane 3). The products were electrophoresed on a high-resolution 6% denaturing polyacrylamide gel. Bands other than the major ones indicated by “EG242” and “EG510” are minor transcription products.
