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. 2024 May 28;14:12267. doi: 10.1038/s41598-024-62783-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Workflow for each differential ultracentrifugation (UC) optimization protocol. (a) Protocols tested included 25 min (UC25min), 48 min (UC48min) and 60 min (UC60min) ultracentrifugation times, carried out to obtain small EVs (SEVs). (b) Protocol in which large EVs (UCLEVs) pelleting was performed. (c) Illustration from UC with washing step (UCwash). Figure was created with BioRender.com.