Table 1.

Values used as input in the centrifugation parameters calculator to predict ultracentrifugation times for UC25min, UC48min and large EVs (LEVs) pelleting from (UCLEVs) protocol.

	Vesicles density [g/cm3]	Urine density [g/cm3]	Urine viscosity [cP]	Rotation speed [g]	Predicted centrifugation time [min]	Complete sedimentation cut-off size [nm]
UC25min	1.18	1.0	1.3076	200,000	19 + 6	40
UC48min	1.08	1.0	1.3076	200,000	42 + 6	40
UCLEVs (LEVs pelleting)	1.15	1.0	1.3076	20,000	8 + 2	210

Values for vesicles density^14,15,31, urine density and viscosity^17,18, predicted centrifugation time¹⁶ and complete sedimentation cut-off size² are represented.