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. 2024 May 28;10:261. doi: 10.1038/s41420-024-02033-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Ruthenium (Ru) concentration was determined by ICP-MS in (A) whole-cell lysates and (B) subcellular fractions nucleus, membrane organelles, cytosol, and cytoskeleton isolated from HT29 cells exposed to 5 µM of indicated Ru-TRZ compounds or DMSO (vehicle control), for 24 h. C Representative confocal images of HT29 cells exposed to 1 µM of indicated fluorescently-tagged Ru-TRZ hybrids (green) or DMSO (vehicle), for 15 min, and stained with mitotracker (red). Nuclei were counterstained with Hoechst 33342 (blue). Scale bar, 25 µm. Results are expressed as Ru concentration (ng/ml) for the mean ± SEM of three independent experiments. Statistical analysis was performed using Student’s t-test and comparing Ru-TRZ-exposed cells with controls. ^#p < 0.05 and ^§p < 0.001 from control cells.