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Sequences of the primers used for the determination of gene expression levels.
| Genes | GenBank No. | Primer Sequence (5′ → 3′) | Length/bp |
|---|---|---|---|
| GAPDH | NM_204305.2 | F: GGTGGCCATCAATGATCCCT | 105 |
| R: CCGTTCTCAGCCTTGACAGT | |||
| Claudin-1 | NM_001013611.2 | F: AGATCCAGTGCAAGGTGTACG | 107 |
| R: AAACACACCAACCAGACCCA | |||
| occludin | NM_205128.1 | F: TGAATGCACCCACTGAGTGTT | 99 |
| R: CCAGAGGTGTGGGCCTTAC | |||
| GATA-3 | NM_001008444.2 | F: CACTTTATCCGCCGGTCACT | 179 |
| R: ACGACTCCAGCTTCATGGTG |
F, forward primer; R, reverse primer.
