Figure 1.

Sex bias in B and T cell phenotypes in ex vivo pediatric tonsils. (A) Tonsil samples from pediatric tonsillectomy patients were evaluated for cell composition by flow cytometry. Tonsil tissues from n=40 donors (19 male, 21 female) were assessed. Created with Biorender. (B) Frequency of total B and T cells. (C) Representative flow cytometry plots showing the distribution of five major B cell subsets. (D) Frequencies of naive (IgD+ CD27-), activated (CD83+), classical memory (CD38- CD27+), and atypical memory (IgD- CD27-) B cells. (E) Distribution of subset-defining markers in classical and atypical memory B cells. (F) Sex-based differences in ex vivo CD4 T cell phenotypes. Each point represents one donor. Mann Whitney U tests followed by multiple hypothesis correction (using the Benjamini & Hochberg method) were used to calculate p values. Boxplots indicate the median value, with hinges denoting the first and third quartiles and whiskers denoting the highest and lowest value within 1.5 times the interquartile range of the hinges.