TABLE 2.
Lipolytic activities of individual fractionsa
| Strain and fraction | Lipolytic activity (U) |
|---|---|
| RN11 | |
| Culture supernatant | 814 |
| Cells (unwashed) | 1,301 |
| Cells (salt washed) | 1,261 |
| FRI1169 | |
| Culture supernatant | 2,480 |
| Cells (salt washed) | 4,140 |
| RN8083 | |
| Culture supernatant | 529 |
| Cells (salt washed) | 4,410 |
| Intracellular | 1,080 |
| Membrane (salt washed) | 3,120 |
a
Densitometry of GML spots (not shown) was performed with the IS-1000 imaging system. Lipolytic activities (obtained from densitometry data after correction for incubation time and sample dilution) are expressed in specific units, each of which corresponds to 1 ng of GML degraded per min ml−1.