REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-phospho-AKT-S473	Cell Signaling Technology	Cat#4051; RRID:AB_331158
Anti-AKT	Cell Signaling Technology	Cat#4685; RRID:AB_2225340
Anti-phospho-AKT-T308	Cell Signaling Technology	Cat#4056; RRID:AB_331163
Anti-p110α	Cell Signaling Technology	Cat#4249; RRID:AB_2165248
Anti-p110β	Cell Signaling Technology	Cat#3011; RRID:AB_2165246
Anti-β-actin	Millipore-Sigma	Cat#A3854; RRID:AB_262011
Chemicals, peptides, and recombinant proteins
Human FGF-basic (FGF-2/bFGF) (154 aa) Animal-Free Recombinant Protein PeproTech®	Thermo Fisher Scientific	Cat#AF-100-18B-1MG
Human EGF, Animal-Free Recombinant Protein, PeproTech®	Thermo Fisher Scientific	Cat#AF-100-15-1MG
AZD6482	AdooQ Bioscience	Cat#A10112
BKM120	AdooQ Bioscience	Cat#A11016
CAL101	AdooQ Bioscience	Cat#A10172
CZC24832	AdooQ Bioscience	Cat#A11363
TGX-221	AdooQ Bioscience	Cat#A10923
BYL719	AdooQ Bioscience	Cat#A11328
Temozolomide	AbMole BioScience	Cat#M2129
Dimethyl sulfoxide	Millipore Sigma	Cat#41639
Puromycin dihydrochloride from Streptomyces alboniger	Millipore Sigma	Cat#P8833
Blasticidin S HCl	Thermo Fisher Scientific	Cat#A1113903
Critical commercial assays
Effectene Transfection Reagent	QIAGEN	Cat#301425
CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)	Promega	Cat#G3582
SuperSignal™ West Pico PLUS Chemiluminescent Substrate	Thermo Fisher Scientific	Cat#34580
SuperSignal™ West Femto Maximum Sensitivity Substrate	Thermo Fisher Scientific	Cat#34096
High Concentration Corning Matrigel Matrix	Corning	Cat#354248
Experimental models: Cell lines
U87MG	ATCC	Cat#HTB-14; RRID:CVCL_XZ66
SF295	Millipore Sigma	Cat#SCC289; RRID:CVCL_1690
LN229	ATCC	Cat#CRL-2611; RRID:CVCL_0393
A172	ATCC	Cat#CRL-1620; RRID:CVCL_0131
NHA human astrocytes	Lonza	Cat#cc-2565
HEK293T	ATCC	Cat#CRL-3216; RRID:CVCL_0063
Experimental models: Organisms/strains
Scid/Beige (C.B-Igh-1b/GbmsTac-Prkdcscid-Lystbg N7)	Taconic Biosciences	Cat#CBSCBG-F; RRID:IMSR_TAC:CBSCBG
Software, algorithms, and public datasets
The Cancer Dependency Map	https://depmap.org	22Q1; Protein_Array; Proteomics; drug_sensitivity
The Cancer Gene Atlas	https://www.cbioportal.org	gbm_tcga
The China Glioma Gene Atlas	http://www.cgga.org.cn/download.jsp	mRNAseq_693
The Genotype-Tissue Expression	https://gtexportal.org	gene_tpm_brain_cortex
The Human Protein Atlas	https://www.proteinatlas.org	Glioma
Plasmids
pBABE-Myr-AKT1	Addgene	Cat#15266
pBABE-Myr-AKT2	Addgene	Cat#9018
pBABE-Myr-AKT3	Addgene	Cat#9019
PIK3CA gRNA	Addgene	Cat#77415
PIK3CB gRNA	Addgene	Cat#76191
PIK3CB gRNA	Addgene	Cat#76192
pLenti-Cas9-Blast	Addgene	Cat#52962
psPAX2	Addgene	Cat#12260
pMD2.G	Addgene	Cat#12259
PIK3CA shRNA RHS4844-101656239	Horizon Discovery	Cat#RHS4012
PIK3CB shRNA RHS4884-10165656350	Horizon Discovery	Cat#RHS4012
PIK3CD shRNA RHS4884-101655755	Horizon Discovery	Cat#RHS4012
Other
Dulbecco’s Modified Eagle Medium	Thermo Fisher Scientific	Cat#12100061
EqualFETALTM bovine serum	Atlas Biologicals	Cat#EF-0500-A
Streptomycin & penicillin, Gibco®	Thermo Fisher Scientific	Cat#15140163
Trypsin-EDTA	Santa Cruz Biotechnology	Cat#sc-363354
AGM™ Astrocyte Growth Medium	Lonza	Cat#cc-3138
Fetal Bovine Serum	Atlas Biologicals	Cat#F-0500-A
MEM Non-Essential Amino Acids Solution	Thermo Fisher Scientific	Cat#11140050
B-27™ Supplement, Gibco®	Thermo Fisher Scientific	Cat#17504001
TrypLE™ Express Enzyme	Thermo Fisher Scientific	Cat#12605010