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. 2024 May 16;40:e00401. doi: 10.1016/j.plabm.2024.e00401

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© 2024 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Flow cytometric and high-performance liquid chromatography analysis of samples from two pregnant women. RBCs were analysed according to the procedures described in Materials and Method section. Gating Strategy for RBC identification, FC dot plots (HbF-PE vs. CA-FITC) divided into Q1, Q2, Q3 and Q4 gates containing fetal RBCs (HbF+, CA-), maternal F-cells (HbF+, CA+), unstained cells (HbF-, CA-) and adult RBCs (HbF-, CA+) and histograms obtained by HPLC analysis (black arrow corresponding to HbF peak) related to a negative (A) and a positive sample (B), respectively.