TABLE 2.
Diet formulation and nutritional composition of experimental diets.
| Ingredients (%) | Control | 0.5AA | 1AA |
|---|---|---|---|
| Corn | 58.55 | 58.03 | 57.55 |
| Soybean meal | 22.00 | 22.00 | 22.00 |
| Limestone a | 9.34 | 9.34 | 9.34 |
| Soybean oil | 3.00 | 3.00 | 3.00 |
| Distiller’s dried grains with soluble | 2.50 | 2.50 | 2.50 |
| Artemisia Annua | 0.00 | 0.50 | 1.00 |
| Glycine | 1.74 | 1.68 | 1.68 |
| Dicalcium phosphate | 1.70 | 1.70 | 1.71 |
| Salt | 0.39 | 0.39 | 0.39 |
| DL-methionine | 0.33 | 0.40 | 0.39 |
| Vitamin premix b | 0.10 | 0.10 | 0.10 |
| L-lysine | 0.10 | 0.10 | 0.11 |
| L-valine | 0.09 | 0.09 | 0.10 |
| L-threonine | 0.08 | 0.09 | 0.10 |
| Mineral mix c | 0.075 | 0.075 | 0.075 |
| Calculated nutritional composition | |||
| ME (kcal/kg) | 2910 | 2910 | 2910 |
| Crude protein (%) | 16.70 | 16.69 | 16.69 |
| Total calcium (%) | 4.00 | 4.00 | 4.00 |
| Available P (%) | 0.45 | 0.45 | 0.45 |
| dLys (%) | 0.80 | 0.80 | 0.80 |
| dMet (%) | 0.54 | 0.54 | 0.57 |
| dTSAA (%) | 0.73 | 0.73 | 0.76 |
| dThr (%) | 0.56 | 0.56 | 0.56 |
| dTrp | 0.16 | 0.16 | 0.16 |
| dArg | 0.89 | 0.89 | 0.89 |
| dVal | 0.71 | 0.70 | 0.70 |
| dILeu | 0.59 | 0.59 | 0.59 |
The level of the fine and coarse limestone ratio was maintained at 50:50.
Vitamin mix provided the following in mg/100 g diet: folic acid, 7.5; riboflavin, 1.5; nicotinic acid amide, 15; vitamin B-12 (source concentration, 0.1%) pyridoxine-HCl, 1.2; d-biotin, 3; thiamine-HCl, 1.5; 2; d-calcium pantothenate 4; menadione sodium bisulfite, 1.98; cholecalciferol (5,000,000 IU/g), 0.09; α-tocopherol acetate (500,000 IU/g), 22.8; retinyl palmitate (500,000 IU/g), 2.8; ethoxyquin, 13.34; dextrose, 762.2; and I-inositol, 2.5.
Mineral mix provided the following in g/100 g diet: CaCO3, 1.48; Ca(H2PO4)2 H2O, 3.62; Na2SeO4, 0.0002; KH2PO4, 1.00; FeSO4 7H2O, 0.05; MnSO4 H2O, 0.035; KIO3, 0.001; MgSO4 7H2O, 0.62; CuSO4·5H2O, 0.008; NaCl, 0.60; ZnCO3, 0.015; 0.0011; KCl, 0.10; and NaMoO4·2H2O, CoCl2 6H2O, 0.00032.