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. 2024 May 15;15:1381548. doi: 10.3389/fphys.2024.1381548

TABLE 2.

Diet formulation and nutritional composition of experimental diets.

Ingredients (%) Control 0.5AA 1AA
Corn 58.55 58.03 57.55
Soybean meal 22.00 22.00 22.00
Limestone a 9.34 9.34 9.34
Soybean oil 3.00 3.00 3.00
Distiller’s dried grains with soluble 2.50 2.50 2.50
Artemisia Annua 0.00 0.50 1.00
Glycine 1.74 1.68 1.68
Dicalcium phosphate 1.70 1.70 1.71
Salt 0.39 0.39 0.39
DL-methionine 0.33 0.40 0.39
Vitamin premix b 0.10 0.10 0.10
L-lysine 0.10 0.10 0.11
L-valine 0.09 0.09 0.10
L-threonine 0.08 0.09 0.10
Mineral mix c 0.075 0.075 0.075
Calculated nutritional composition
ME (kcal/kg) 2910 2910 2910
Crude protein (%) 16.70 16.69 16.69
Total calcium (%) 4.00 4.00 4.00
Available P (%) 0.45 0.45 0.45
dLys (%) 0.80 0.80 0.80
dMet (%) 0.54 0.54 0.57
dTSAA (%) 0.73 0.73 0.76
dThr (%) 0.56 0.56 0.56
dTrp 0.16 0.16 0.16
dArg 0.89 0.89 0.89
dVal 0.71 0.70 0.70
dILeu 0.59 0.59 0.59
a

The level of the fine and coarse limestone ratio was maintained at 50:50.

b

Vitamin mix provided the following in mg/100 g diet: folic acid, 7.5; riboflavin, 1.5; nicotinic acid amide, 15; vitamin B-12 (source concentration, 0.1%) pyridoxine-HCl, 1.2; d-biotin, 3; thiamine-HCl, 1.5; 2; d-calcium pantothenate 4; menadione sodium bisulfite, 1.98; cholecalciferol (5,000,000 IU/g), 0.09; α-tocopherol acetate (500,000 IU/g), 22.8; retinyl palmitate (500,000 IU/g), 2.8; ethoxyquin, 13.34; dextrose, 762.2; and I-inositol, 2.5.

c

Mineral mix provided the following in g/100 g diet: CaCO3, 1.48; Ca(H2PO4)2 H2O, 3.62; Na2SeO4, 0.0002; KH2PO4, 1.00; FeSO4 7H2O, 0.05; MnSO4 H2O, 0.035; KIO3, 0.001; MgSO4 7H2O, 0.62; CuSO4·5H2O, 0.008; NaCl, 0.60; ZnCO3, 0.015; 0.0011; KCl, 0.10; and NaMoO4·2H2O, CoCl2 6H2O, 0.00032.