TABLE 1.
Bacterial strains, plasmids, and oligonucleotides used in this study
| Strain, plasmid, or oligonucleotide | Relevant features | Reference |
|---|---|---|
| S. pneumoniae | ||
| R6 | Subclone of R36A, derivative of D39 | 34 |
| KNR.7/87 | Serotype 4 clinical isolate; TIGR sequence | 1 |
| D39 | Serotype 2; NCTC 7466 | 3 |
| 1 | Clinical isolate; Roche strain collection | 20 |
| 1711 | Clinical isolate; Roche strain collection | 20 |
| 4241 | ATCC reference strain | P. Geslin (Paris, France) |
| LSP22 | R6 hk13′::pAS1::′hk13 | This study |
| Plasmids | ||
| pJDC9 | Vector (Ery); nonreplicative in S. pneumoniae | 7 |
| pAS1 | Ery; pJDC9 derivative | 27 |
| pRPL54 | pAS1 derivative carrying a 401-bp fragment targeting hk13 (nt 456–857) | This study |
| Oligonucleotides | ||
| HK13EF | GAGAAAGGGAATTCTCGATAAAGC | This study |
| HK13RB | CTGACTGGGATCCTATAAGACGGAGa | This study |
a
Underlined nucleotides were modified to create an in-frame stop codon and an EcoRI restriction site.