TABLE 4.
Metabolic enzymes involved in aspartylaspartate degradation by P. gingivalis ATCC 33277
| Enzyme | Substrate | Electron carrier | Sp act (U/g of protein)a |
|---|---|---|---|
| Aspartylaspartate dipeptidase | Aspartylaspartate | 64.1 ± 10.5 | |
| Aspartate ammonia-lyase | Aspartate | 402 ± 242 | |
| Aspartate aminotransferase | Aspartate and 2-oxoglutarate | 3.19 ± 5.52 | |
| Fumarase | Fumarate | 40.5 ± 7.17 | |
| Malate dehydrogenase | Oxaloacetate | NADH | 390 ± 96.9 |
| NADPH or MVredb | ND | ||
| Oxaloacetate decarboxylase | Oxaloacetate | 986 ± 190 | |
| Pyruvate oxidoreductase | Pyruvate and CoA | NAD or NADP | ND |
| MVoxc | 672 ± 435 | ||
| Fumarate reductase | Fumarate | NADH or NADPH | ND |
| MVred | 21.4 ± 5.52 |
a
Mean ± standard deviation obtained from three independent experiments. ND, not detected.
b
MVred, reduced methylviologen.
c
MVox, oxidized methylviologen.