TABLE 5.
Metabolic enzymes involved in acyl-CoA metabolism, ATP formation, and oxidation-reduction of NAD(P) and methylviologen by P. gingivalis ATCC 33277
| Enzyme | Substrate | Electron carrier | Sp act (U/g of protein)a |
|---|---|---|---|
| Acyl-CoA:acetate CoA-transferase | Butyryl-CoA and acetate | 100 ± 7.07 | |
| Propionyl-CoA and acetate | 154 ± 17.8 | ||
| Succinyl-CoA and acetate | 80.7 ± 16.2 | ||
| Phosphotransacetylase | Acetyl-CoA and inorganic phosphate | 4.83 ± 2.35 | |
| Acetate kinase | Acetate and ATP | 9.42 ± 5.07 | |
| Methylviologen:NAD(P) oxidoreductase | NADH and MVoxb | 510 ± 123 | |
| NADPH and MVox | 65.8 ± 19.3 |
Mean ± standard deviation obtained from three independent experiments.
MVox, oxidized methylviologen.