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. 2024 Apr 23;15(11):3297–3312. doi: 10.7150/jca.95549

Figure 5.

Figure 5

Knockdown of ACAT1 downregulated the AKT/GSK3β signaling pathway. (A) GSEA of the UROMOL cohort data showed enrichment of the PI3K/AKT/mTOR signaling pathway. (B) Spearman correlation analysis between ACAT1 and pathway scores. The ordinate represents the pathway score, and the abscissa represents gene expression. (C) Immunoblotting was performed to detect changes in the expression of AKT/GSK3β signaling pathway-related proteins after knockdown (UM-UC-3 and T24 cells) or overexpression (5637 and T24 cells) of ACAT1. (D-E) Statistical analysis of the changes in the AKT and GSK3β mRNAs detected by qRT-PCR after knockdown of ACAT1 in UM-UC-3 and T24 cells, ns: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001.