Figure 4.

Downregulation of human Hsp70A1A by siRNA in erythroid progenitor cells (EPCs)

(A i, ii, iii) Expression of human Hsp70A1A was detected by IFA in wild-type (WT) EPCs (i) and those transfected with siRNA targeting Hsp70A1A mRNA at 100 nM (ii) and 250 nM concentration (iii). DIC: differential interference contrast image, DAPI: nuclear staining 40, 6-diamidino-2-phenylindole (blue); Hsp70A1A: mouse anti-Hsp70A1A (green); Scale bar: 2 μm (A iv) Intensity of Hsp70A1A staining in EPCs under each condition is shown as an intensity graph (p < 0.0001∗∗∗∗).

(B i) Graph representing percentage invasion of malaria parasite in EPCs and those transfected with siRNA targeting Hsp70A1A mRNA at 100 nM and 250 nM concentration. The graph is the average of triplicate values and standard deviation is represented by error bars. (B ii) Giemsa-stained images of Pf3D7 in wild-type (WT) EPCs and those transfected with siRNA targeting Hsp70A1A mRNA at 100 nM and 250 nM concentration. Scale Bar represents 5 μm.