Antibodies |
|
Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 |
InvitrogenTM
|
Cat # A-11001; RRID: AB_2534069 |
Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 |
InvitrogenTM
|
Cat # A-11012; RRID: AB_2534079 |
Mouse anti-Histidine primary monoclonal Antibody |
Thermoscientific |
Cat # PA1-983B; RRID: AB_1069891 |
Rabbit anti-Mouse IgG1 Secondary Antibody, HRP |
Thermoscientific |
Cat # PA1-86329; RRID: AB_933678 |
GAPDH-HRP conjugated |
Abclonal |
Cat # AC035; RRID: AB_2769863 |
|
Chemicals, peptides, and recombinant proteins |
|
RPMI Medium 1640 |
GibcoTM
|
Cat # 23400013 |
2-Phenylethyl beta-D-thiogalactoside |
Sigma-Aldrich |
Cat #P4902 |
5-Bromo-4chloro-3indoxyl-beta-D-galactopyronoside (X-gal Substrate) |
G-Biosciences |
Cat #R1233 |
Bovine Serum Albumin, BSA |
Sisco Research Laboratory (SRL) |
Cat # 97350 |
Freund’s Complete Adjuvants |
Sigma-Aldrich |
Cat #F5881 |
Freund’s incomplete Adjuvants |
Sigma-Aldrich |
Cat #F5506 |
o-Nitrophenyl-beta-D-Galactopyranoside (o-NPG) |
Sigma-Aldrich |
Cat # 45-N1127 |
Ni-NTA Resin |
G-Biosciences |
Cat # 786-90 |
Precision plus protein dual color standard |
Bior-Rad |
Cat # 1610374 |
N-Acetyl-D-glucosamine |
Sigma-Aldrich |
Cat # A3286 |
ProLong™ Gold Antifade Mountant with DAPI |
InvitrogenTM
|
Cat #P36935
|
Arabinose |
Sisco Research Laboratory (SRL) |
Cat # 52392 |
IPTG |
Thermoscientific |
Cat #R0392 |
Protein Assay Dye |
Bio-Rad |
Cat # 5000006 |
|
Critical commercial assays |
|
TMB substrate kit |
Thermoscientific |
Cat # 34021 |
ECL Western Blotting Substrates kit |
Bio-Rad |
Cat # 1705060 |
Monolith Protein Labeling Kit RED-NHS 2ND Generation (Amine Reactive) |
nanoTEMPER |
Cat # MO-L011 |
|
Experimental models: Organisms/strains |
|
Plasmodium falciparum 3D7 strain |
Malaria Research and Reference Reagent Resource Center (MR4) |
NA |
|
Software and algorithms |
|
ImageJ |
NIH |
https://imagej.nih.gov/ij; RRID: SCR_003070 |
Graphpad Prism |
GraphPad Prism Software |
https://www.graphpad.com/scientific-software/prism; RRID: SCR_002798 |
Biorender |
NA |
https://www.biorender.com/; RRID: SCR_018361 |