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. 2024 May 7;27(6):109918. doi: 10.1016/j.isci.2024.109918
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 InvitrogenTM Cat # A-11001; RRID: AB_2534069
Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 InvitrogenTM Cat # A-11012; RRID: AB_2534079
Mouse anti-Histidine primary monoclonal Antibody Thermoscientific Cat # PA1-983B; RRID: AB_1069891
Rabbit anti-Mouse IgG1 Secondary Antibody, HRP Thermoscientific Cat # PA1-86329; RRID: AB_933678
GAPDH-HRP conjugated Abclonal Cat # AC035; RRID: AB_2769863

Chemicals, peptides, and recombinant proteins

RPMI Medium 1640 GibcoTM Cat # 23400013
2-Phenylethyl beta-D-thiogalactoside Sigma-Aldrich Cat #P4902
5-Bromo-4chloro-3indoxyl-beta-D-galactopyronoside (X-gal Substrate) G-Biosciences Cat #R1233
Bovine Serum Albumin, BSA Sisco Research Laboratory (SRL) Cat # 97350
Freund’s Complete Adjuvants Sigma-Aldrich Cat #F5881
Freund’s incomplete Adjuvants Sigma-Aldrich Cat #F5506
o-Nitrophenyl-beta-D-Galactopyranoside (o-NPG) Sigma-Aldrich Cat # 45-N1127
Ni-NTA Resin G-Biosciences Cat # 786-90
Precision plus protein dual color standard Bior-Rad Cat # 1610374
N-Acetyl-D-glucosamine Sigma-Aldrich Cat # A3286
ProLong™ Gold Antifade Mountant with DAPI InvitrogenTM Cat #P36935
Arabinose Sisco Research Laboratory (SRL) Cat # 52392
IPTG Thermoscientific Cat #R0392
Protein Assay Dye Bio-Rad Cat # 5000006

Critical commercial assays

TMB substrate kit Thermoscientific Cat # 34021
ECL Western Blotting Substrates kit Bio-Rad Cat # 1705060
Monolith Protein Labeling Kit RED-NHS 2ND Generation (Amine Reactive) nanoTEMPER Cat # MO-L011

Experimental models: Organisms/strains

Plasmodium falciparum 3D7 strain Malaria Research and Reference Reagent Resource Center (MR4) NA

Software and algorithms

ImageJ NIH https://imagej.nih.gov/ij; RRID: SCR_003070
Graphpad Prism GraphPad Prism Software https://www.graphpad.com/scientific-software/prism; RRID: SCR_002798
Biorender NA https://www.biorender.com/; RRID: SCR_018361