| Component | Amount (μl) | Final (including sample) |
|---|---|---|
| H2O | 59.5 | - |
| 10× PNK7 buffer | 10.0 | 1.05× |
| 1 M DTT | 0.5 | 5.26 mM |
| Turbo DNase | 1.0 | 2 U |
| T4 PNK enzyme | 4.0 | 40 U |
| Total | 75.0 | - |
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