TABLE 3.
Percentage of in vitro-cleaved transcripts containing helix 9 of 23S rRNA using E. coli and R. capsulatus RNases III at 130 mM and 250 mM KCla
| Strain (lane nos. in Fig. 3) | % Transcript cleaved in vitro using:
|
|||
|---|---|---|---|---|
| RNase IIIEc with:
|
RNase IIIRc with:
|
|||
| 130 mM KCl | 250 mM KCl | 130 mM KCl | 250 mM KCl | |
| R. sphaeroides WS8 (21–25) | 0 | 0 | 98 | 43 |
| R. sphaeroides 17023 (56–58) | 0 | 0 | 90 | 10 |
| R. capsulatus B10 (46–50) | 0 | 0 | 97 | 12 |
| R. capsulatus 37b4 (6–10) | 0 | 0 | 94 | 14 |
| R. palustris 5D (1–5) | 96 | 75 | 100 | 99 |
| B. henselae ATCC 49882 (11–15) | 97 | 70 | 99 | 96 |
| S. fredii MSDJ 1536 (31–35) | 2 | 1.5 | 100 | 100 |
| R. giardinii H152 (26–30) | 25 | 0 | 100 | 80 |
| R. etli CFN42 (16–20) | 10 | 0 | 99 | 99 |
| R. etli Viking I (41–45) | 80 | 0 | 98 | 99 |
| R. gallicum R602 (36–40) | 60 | 0 | 100 | 86 |
| R. leguminosarum ATCC 10004 (51–55) | 88 | 2 | 99 | 48 |
a
The reported values are the average of two experiments; the values did not deviate by more than 15%.