TABLE 4.
Summary of the approximate lengths of the helix 9 containing transcripts from the strains studied here and the approximate lengths of the fragments arising after in vitro cleavage of these transcripts by RNases III from R. capsulatus and E. colia
| Transcript source strain (lane nos. in Fig. 3) | Approximate length (no. of nt) of:
|
|||
|---|---|---|---|---|
| Full-length transcript | 5′-End fragment | Internal fragment | 3′-End fragment | |
| R. sphaeroides WS8 (21–25) | 180 | 40 | 50 | 90 |
| R. sphaeroides 17023 (56–58) | 145 | 55 (40) | – (15) | 90 |
| R. capsulatus B10 (46–50) | 145 | 55 (40) | – (15) | 90 |
| R. capsulatus 37b4 (6–10) | 200 | 110 (40) | – (70) | 90 |
| R. palustris 5D (1–5) | 170* | 40 | 40 | 90 |
| B. henselae ATCC 49882 (11–15) | 160* | 40** | 30** | 90** |
| S. fredii MSDJ 1536 (31–35) | 160 | 40 | 30 | 90 |
| R. giardinii H152 (26–30) | 160 | 40 | 30 | 90 |
| R. etli CFN 42 (16–20) | 260* | 40** | 130 (processed in fragments of 30–40 nt) | 90** |
| R. etli Viking I (41–45) | 250* | 40** | 120 (processed in fragments of 30–40 nt) | 90** |
| R. gallicum R602 (36–40) | 310* | 40** | 180 (processed further) | 90** |
| R. leguminosarum ATCC 10004 (51–55) | 250* | 40** | 120** | 90** |
a
In some of the Rhodobacter strains, the 3′-cleavage site was used as a primary processing site, and only a small amount of the 5′ fragment is further processed at the secondary, 5′-processing site. The length of the resulting 5′ and internal fragments is given in parentheses. ∗, differences in the cleavage sites of both enzymes; ∗∗, multiple fragments which differ in their length by a few nucleotides due to cleavage of different scissile bonds at the 5′- and/or 3′-processing sites.