TABLE 3.
Characterization of the representatives of transposon mutants
| Cosmid | Insertion sitea (bp) | Mutated gene | Adipic acid productionb | Major metabolitec | Function of mutated gened |
|---|---|---|---|---|---|
| 8F6 parent | None | None | + | Adipic acid | N/A |
| 8F6ty1 | 8925 | chnR | − | Cyclohexanol | Transcriptional activator |
| 8F6ty2 | 11990 | chnA | − | Cyclohexanol | Cyclohexanol dehydrogenase |
| 8F6ty9 | 7370 | chnE | +/− | 6-hydroxy hexanoic acid | 6-Oxohexanoic acid dehydrogenase |
| 8F6ty18 | 6070 | chnB | − | Cyclohexanone | Cyclohexanone monooxygenase |
| 8F6ty24 | 15510 | chnC | − | Caprolactone | Caprolactone hydrolase |
| 8F6ty25 | Vector | Vector | + | Adipic acid | Not essential |
| 8F6ty26 | 10030 | chnZ | + | Adipic acid | Not essential |
| 8F6ty29 | 11390 | chnY | + | Adipic acid | Not essential |
| 8F6ty38 | 13950 | chnX | + | Adipic acid | Not essential |
| 8F6ty95 | 15180 | chnD | − | 6-hydroxy hexanoic acid | 6-Hydroxyhexanoic acid dehydrogenase |
| Vector | None | None | − | Cyclohexanol | N/A |
a
Location in reference to the 17,417-bp contig.
b
Conversion of cyclohexanol to adipic acid was assayed by HPLC. +, production; −, no production; ±, decreased production.
c
Metabolites of cyclohexanol were detected by GC/MS.
d
N/A, does not contain mutated gene; not essential, not required for conversion of cyclohexanol to adipic acid.