Figure 1.

Strategy for the enrichment of malonylated peptides from a solution of proteolytic peptides

After protein reduction, alkylation, trypsin digestion, and proteolytic peptide HLB desalting, 100-μg of the peptide solution is aliquoted for protein-level analysis to identify protein changes and to normalize malonylated peptide abundance. The remaining material is used to enrich the malonylated peptides by immunoprecipitation using antibody-coated beads. The resulting enrichment samples are used to determine malonyl-level changes.