Figure 3.

Malonyl-lysine analysis in K562 cells by data-independent acquisition-mass spectrometry (DIA-MS)

(A) Retention time calibration obtained for a replicate of the malonyl enrichment DIA data set.

(B) Relative identification count of the modifications set for the DIA search.

(C) Content of the spectral library generated in Spectronaut from the DIA acquisitions of the malonyl enrichment samples.

(D) Pseudo-MS/MS spectrum of the PDPA⁵KmalSAPAPK peptide (m/z 582.80, z = 2+) (histone H2B2F) contained in the spectral library and visualized in Skyline.

(E) Extracted ion chromatogram of the PDPA⁵KmalSAPAPK peptide (m/z 582.80, z = 2+) (histone H2B2F) visualized in Skyline.