Figure 4.

CRISPRi K562 cell purification by FACS and gene knockdown efficiency examination by qPCR and western blotting

(A) sgRNA-expressing cells were BFP (+), and collected after FACS. Gating strategies are shown here. The percentage of BFP (+) cells were about 14–27% of live cells. FSC, forward scatter; SSC, side scatter; BFP, blue fluorescent protein.

(B) Bar graph shows the target RNA expression level in the knockdown K562 cell line (sgTarget) relative to the scramble sgRNA-expressing negative control (sgNC). The lower the percentage, the higher the knockdown efficiency.

(C) Western blotting results showing the protein expression levels of KATs and SIRT5 in knockdown cell lines.