Table 3.
Component | Vol. |
---|---|
DNA | 1 μg (can be scaled up) |
rCutSmart (10 ×) | 5 μL |
BlpI | 1 μL (10 units) |
Nuclease-free Water | to 50 μL |
Component | Vol. |
---|---|
DNA | 1 μg (can be scaled up) |
rCutSmart (10 ×) | 5 μL |
BlpI | 1 μL (10 units) |
Nuclease-free Water | to 50 μL |