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. 2024 May 20;5(2):103074. doi: 10.1016/j.xpro.2024.103074

Table 3.

BlpI digestion mixture

Component Vol.
DNA 1 μg (can be scaled up)
rCutSmart (10 ×) 5 μL
BlpI 1 μL (10 units)
Nuclease-free Water to 50 μL