Table 4.
Ligation mixture
| Component | Vol. |
|---|---|
| 1:20 diluted annealed oligos | 1 μL |
| Linearized backbone vector | 50 ng |
| Quick Ligase Reaction Buffer (2 ×) | 5 μL |
| Quick Ligase | 0.5 μL |
| Nuclease-free Water | to 10 μL |
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