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. 2024 May 20;5(2):103074. doi: 10.1016/j.xpro.2024.103074

HPLC solvent B

Reagent Final concentration Amount
Acetonitrile 98% 490 mL
LC-MS-grade water 2% 10 mL
Formic acid 0.1% 500 μL
Total N/A 500 mL

Note on storage conditions: HPLC Solvents A and B can be stored at 20°C–25°C for up to a year.