FACS buffer

Reagent	Final concentration	Amount
PBS (1 ×)	1 ×	9760 μL
FBS	2%	200 μL
EDTA (0.5 M)	2 mM	40 μL
Total	N/A	10 mL

Note on storage conditions: FACS buffer can be stored at 4°C for a week after preparation.