Puromycin selection RPMI 1640 medium

Reagent	Final concentration	Amount
RPMI 1640 (1 ×)	1 ×	44.5 mL
FBS	10%	5 mL
Pen-Strep (100 ×)	1 ×	500 μL
Puromycin (10 mg/mL)	1 μg/mL	5 μL
Total	N/A	50 mL

Note on storage conditions: Cell culture media can be stored at 4°C for a week after preparation.